In situ hybridization (ISH) is a type of hybridization that uses a labeled complementary DNA, RNA or modified nucleic acids strand (i.e., probe) to localize a specific DNA or RNA sequence in a portion or section of tissue (in situ), or, if the tissue is small enough (e.g., plant seeds, Drosophila embryos), in the entire tissue (whole mount ISH), in cells, and in circulating tumor cells (CTCs).

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Advances in RNA in situ hybridization transform molecular detection with morphological context enabling new applications. Scientists use RNA ISH to extract data dimensions.

Immunohistochemistry (IHC) and RNA in situ Hybridization are widely used technologies sharing the unique capacity to analyze a marker at the single cell level while preserving the morphological context. In different situations, IHC and ISH are used in conjunction to validate data or provide complementary information.

In situ hybridization (ISH) is a powerful technique for localizing specific nucleic acid targets within fixed tissues and cells, allowing users to obtain temporal and spatial information about gene expression and genetic loci. While the basic workflow of ISH is similar to that of blot hybridizations-the nucleic acid probe is synthesized, labeled, purified, and annealed with the specific target-the difference is the greater amount of information gained by visualizing the results within the tissue.

The global market for In Situ Hybridization (ISH) at $4.3 billion in 2017 is anticipated to reach $7.8 billion by 2024 2017. CAGR of 7.8% in the next five years (2017 to 2024). Increasing diagnosis and growing incidence & prevalence of cancer, technology advancements in therapeutics, increasing government initiatives globally are expected to drive the growth of the market in the coming years.

Companies Profiled:

Market Leaders:

• Leica Biosystems Nussloch GmbH

• Thermo Fisher Scientific

• F. Hoffmann-La Roche AG

• Abbott Laboratories

• Agilent Technologies

• PerkinElmer

• Qiagen / Exiqon A/S

• Merck KGaA

• BioGenex Laboratories

• BioTechne / Advanced Cell Diagnostics (ACD)

Key Topics:

• In Situ Hybridization

• Molecular Diagnostics

• Cervical Cancer Stain

• Fish Steps Reduction

• Fluorescence In Situ Hybridization (FISH)

• In Situ Hybridization (ISH)

• FISH Probe Kit

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